Table 2.
Comparison of detection of respiratory virus by three automatic extraction methods from nasopharyngeal swab specimens.
| Virus | QIAcube | EZ1 advanced XL | MICROLAB Nimbus IVD | Total number of specimens |
|---|---|---|---|---|
| RV | + | + | + | 14 |
| + | − | + | 3 | |
| + | − | − | 1a | |
| − | + | + | 1 | |
| − | + | − | 2b | |
|
| ||||
| MPV | + | + | + | 7 |
| + | + | − | 1 | |
|
| ||||
| PIV3 | + | + | + | 8 |
|
| ||||
| PIV1 | + | + | + | 1 |
| − | + | − | 1b | |
|
| ||||
| PIV4 | + | − | − | 1b |
|
| ||||
| RSV-A | + | + | + | 7 |
| − | + | − | 1b | |
|
| ||||
| ADV | + | + | + | 2 |
| − | + | + | 1 | |
| + | + | − | 2 | |
| − | + | − | 3b | |
|
| ||||
| FluB | + | + | + | 2 |
| − | + | − | 1a | |
|
| ||||
| FluA | + | + | + | 2 |
|
| ||||
| BoV | + | + | + | 1 |
| + | − | − | 1a | |
|
| ||||
| EV | + | + | + | 2 |
| + | − | − | 1b | |
|
| ||||
| CoV-OC43 | 1 | 1 | 1 | 1 |
|
| ||||
| CoV-NL63 | − | − | + | 1b |
|
| ||||
| CoV-229E | − | − | + | 1b |
|
| ||||
| Total | 57 | 59 | 54 | 68 |
aVirus detected by only 1 of the three automated nucleic acid extraction systems, and we confirmed this result by sequencing analysis and confirmed it as “true positive.”
bVirus detected by only 1 of the three automated nucleic acid extraction systems, but they were not detected by repeated test with the Seeplex RV15 ACE Detection kit (Seegene).