(A) PFO binding to liposomes composed of POPC (—), 5% 25-HC
in POPC (−–−), or 5% 27-HC in POPC (···)
with varying concentrations of cholesterol. (B) Mean POPC lipid surface
area in simulated membrane bilayers containing no additional sterols,
an additional 30 mol % cholesterol, an additional 30 mol % 25-HC (···),
or an additional 30 mol % 27-HC. Asterisks show p values of <0.01 for the difference from the control POPC simulation,
as calculated with Kolmogorov–Smirnof tests. (C) PFO binding
to liposomes composed of DOPC (—) or 5% 7α-HC in DOPC
(−–−), with varying concentrations of cholesterol.