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. Author manuscript; available in PMC: 2014 May 14.
Published in final edited form as: Sci Signal. 2009 Nov 24;2(98):ra75. doi: 10.1126/scisignal.2000559

Fig. 5.

Fig. 5

Rapamycin rejuvenates HSC from old mice. A. Bone marrow cellularity of tibia and femurs of young (Y, 2 months of age) mice, or age-matched, 22–24 months old mice treated with either vehicle (O-veh) or old mice treated with rapamycin (O-rapa). n=5. B. The percentage of LSK and FLSKCD150/48/34 HSC populations in bone marrow of Y, O-veh and O-rapa mice. n=5. C. BrdU incorporation. Left panels show representative profiles of BrdU incorporation in HSCs, and right panels show Mean ± SD of BrdU+ cells (n=4). D. HSCs were sorted by FACS and p16Ink4a and p19Arf mRNA abundance was measured by real-time PCR and normalized to Hprt abundance; values shown are mean ± SD (n=3). E. Diagram of experiments. F. Hematopoietic function of HSC as measured by CD45 congenic markers on leukocytes of recipient peripheral blood at 4 weeks and 28 weeks after transplantation. The left panels show the % of donor-type cells among leukocytes (CD45+) and right panels show the reconstitution rates of B220+ (B), CD3+ (T) and Mac-1+ (M) lineages. Data shown were from three independent experiments with 15 recipients per group.