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. Author manuscript; available in PMC: 2014 May 14.
Published in final edited form as: Chem Biol. 2007 May;14(5):565–576. doi: 10.1016/j.chembiol.2007.04.006

Figure 1.

Figure 1

Inactivation of NRPS presumably involved in aeruginosin biosynthesis genes by homologous recombination and HPLC analysis of wild type and mutant.

(A) Schematic representation of ΔNRPS-d-Leu knock-out plasmids for the insertional mutagenesis of P. agardhii CYA126/8. The chloramphenicol resistance gene cassette (Cmr) is highlighted in black. (B) PCR amplification with the DNA from wild type (WT) and ΔNRPS-d-Leu mutant (MT) with primers amplifying the region flanking the insertion site. (C) HPLC-DAD profile of extract from Planktothrix agardhii CYA 126/8 wild type. D) HPLC-DAD profile of extract from ΔNRPS-d-Leu mutant. The peaks at min 9.07 (1) and 10.56 (2) represent putative aeruginosins, the peaks at min 26.6 (3) and 33.8 (4) [d-Asp]-microcystin-RR and [d-Asp]-microcystin-LR, respectively.