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. Author manuscript; available in PMC: 2014 May 14.
Published in final edited form as: Chem Biol. 2007 May;14(5):565–576. doi: 10.1016/j.chembiol.2007.04.006

Figure 4.

Figure 4

Analysis of putative Choi biosynthesis genes.A-B) Inactivation of putative Choi biosynthesis genes by homologous recombination. (A) Schematic representation of ΔaerEF and ΔaerD knock-out plasmids for the insertional mutagenesis of P. agardhii CYA126/8. Chloramphenicol resistance gene cassettes (Cmr) are highlighted in black.

(B) PCR amplification with the DNA from wild type (lanes 1 and 4), ΔaerEF and ΔaerD mutants (lanes 2 and 5), and ΔaerEF and ΔaerD plasmid constructs (lanes 3 and 6) with primers amplifying the aerD-aerF (lanes 1-3) and the aerD (lanes 4-6) regions, respectively. C-G) LC-ESI MS (Positive SIM, range m/z 714.80-715.80) of extracts from (C) P. agardhii CYA126/8 WT, (D) P. agardhii CYA126/8 ΔAerEF, (E) P. agardhii CYA126/8 ΔAerEF + Choi, (F) P. agardhii CYA126/8 ΔAerD, (G) P. agardhii CYA126/8 ΔAerD + Choi. 1; aeruginoside 126A.