Table 2.
Deduced Functions of ORFs in the Aeruginoside Biosynthetic Gene Cluster
| Protein | Amino Acids | Deduced Function | Sequence Similarity | Identity/SimilarityAccession Number (AA length) |
|---|---|---|---|---|
| AerA | 1416 | PKS (A KR ACP) | BarE (Lyngbya majuscula) | 44%/64% (587)AAN32979 |
| AerB | 1598 | NRPS (C A T E) | NosC (Nostoc sp. GSV224) | 54%/71% (944)AAF17280 |
| AerC | 736 | Oxygenase | Putative diaminopimelate decarboxylase (Burkholderia mallei 10229) |
30%/49% (707)ZP00431845 |
| AerD | 202 | Decarboxylase | BacA (Bacillus amyloliquefaciens AAM90573) | 39%/58% (191)AAM90573 |
| AerE | 213 | Unknown | BacB (Bacillus subtilis) | 26%/45% (208)AAM90569 |
| AerF | 264 | Reductase | BacC (Bacillus subtilis) | 36%/55% (256)AA65204 |
| AerG | 1622 | NRPS (C A T C T) | McyB (Microcystis aeruginosa) | 47%/64% (927)AAE09608 |
| AerH | 308 | Dioxygenase | Thymine dioxygenase (Rhodotorula glutinis) | 30%/50% (517)AAU12179 |
| AerI | 418 | Glycosyltransferase | Spore coat protein SA (Bacillus subtilis) | 26%/46% (336)P46915 |
|
| ||||
| ORF1 | 248 | Circadian clock protein | KaiA (Synechococcus elongatus) | 31%/51% (227)1R8JA |
| ORF2 | 126 | Unknown | no similarity | |
| ORF3 | 288 | Unknown | no similarity | |
| ORF4 | 229 | Unknown | no similarity | |
| ORF5 | 170 | Unknown | Putative reverse transcriptase (Nostoc sp. PCC7120) | 70%/83% (157) BAB77479 |
| ORF6 | 428 | Unknown | Retron-type reverse transcriptase (Anabaena variabilis ATCC29413) |
58%/74% (415)ABA24728 |
| ORF7 | 386 | Oxidoreductase | Putative aldo/keto reductase (Tricodesmium erythraeum IMS101) |
70%/84% (383)ZP00328942 |
| ORF8 | 339 | Sulfotransferase | Putative sulfotransferase (Shewanella sp. PV-4) | 22%/39% (228)EAP04083 |
| ORF9 | 664 | ABC-type transporter | NcpC (Nostoc sp. ATCC 53789) | 57%/75% /663)AAO23332 |
| ORF10 | 257 | Succinate dehydrogenase | Succinate dehydrogenase (Sulfolobus tokodaii) | 33%/53% (238)BAB40685 |