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. 2014 May 14;9(5):e95914. doi: 10.1371/journal.pone.0095914

Figure 1. Genomic fragments from high copy screen suppress Htt103Q-NLS toxicity.

Figure 1

(A) Genomic fragments (GF) 1 through 12 suppress Htt103Q-NLS toxicity as monitored by growth assay plated in 5-fold dilutions on galactose containing media. (B) Impact of GF1-12 upon Htt103Q-NLS expression levels as monitored by Western blot. (C) Impact of GF1-12 upon aggregation of Htt103Q-NLS as monitored by fluorescence microscopy. Bar graph indicates percent of cells with Htt103Q-NLS found in intranuclear foci. Each bar indicates the average ± sd of at least 100 cells counted in at least 3 different experiments. Cultures for Western blotting and microscopy were obtained by inducing Htt103Q-NLS with 2% galactose for 4–5 h.