Figure 4. Alignment of T. cruzi, T. brucei, M. tuberculosis and human Lanosterol 14-α demethylases, showing the non-synonymous changes identified in this work (red arrows).

Important residues either in Tc14DM or in the CYP51 family are noted [100], [101], as well as residues associated with resistance to azoles in C. albicans and U. necator [56], [57]. PS00086 is the Prosite Cytochrome 450 motif (cysteine heme-iron ligand signature).