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. Author manuscript; available in PMC: 2014 May 14.
Published in final edited form as: Chem Biol. 2009 Dec 24;16(12):1230–1239. doi: 10.1016/j.chembiol.2009.10.014

Fig. 4.

Fig. 4

Biochemical properties of M. tuberculosis IspE. a. The optimal pH for catalytic activity was determined using MES (pH 5.5 - pH 7.0), MOPS (pH 7.0 - pH 7.5), Tris (pH 7.5 - pH 8.5), TAPS (pH 8.5 - pH 9.0), and CAPS (pH 9.0 - pH 10.5) using appropriate counter ions. b. Divalent cations (Mg2+, Mn2+, Zn2+, or Ca2+) were added to the reaction mixtures at the indicated concentrations. The reaction mixtures were as described in Experimental Procedures.