Table 1. The propensity scores of amino acids to be a bioluminescent protein (BLP) and amino acid composition (%) using BLP-TRN.

Amino acid	BLP Score (rank)	Composition of BLP: A (%)	Composition of Non-BLP: B (%)	Composition difference: A–B (%)
G-Gly	573.025 (1)	7.53	6.49	1.04
C-Cys	572.850 (2)	2.09	1.28	0.81
F-Phe	548.075 (3)	4.58	3.90	0.68
H-His	534.450 (4)	2.75	2.33	0.42
Y-Tyr	508.750 (5)	3.56	3.05	0.51
D-Asp	498.100 (6)	5.82	5.55	0.27
M-Met	483.150 (7)	2.42	2.27	0.15
V-Val	477.775 (8)	6.60	6.45	0.15
P-Pro	472.950 (9)	4.99	4.91	0.08
I-Ile	456.525 (10)	5.60	5.66	–0.05
T-Thr	452.750 (11)	5.51	5.40	0.11
N-Asn	451.125 (12)	4.23	4.21	0.02
Q-Gln	435.025 (13)	3.70	4.03	–0.33
W-Trp	434.575 (14)	1.22	1.27	–0.05
L-Leu	426.700 (15)	9.31	9.70	–0.39
E-Glu	404.075 (16)	6.25	6.79	–0.54
R-Arg	384.875 (17)	4.96	5.51	–0.55
S-Ser	368.500 (18)	6.61	7.27	–0.66
A-Ala	359.750 (19)	7.12	8.15	–1.03
K-Lys	336.275 (20)	5.14	5.80	–0.66
R	1.00	–0.30	–0.50	0.97

The total number of amino acids in the used datasets of BLPs and non-BLPs is 130,904 and 120,607, respectively.