Figure 1. In vitro derivation of CD34+ HSC/HPC from Human iPSC.

Human iPSC (hiPSC-19 and hiPSC-21; prepared as illustrated in Figure 1) were cultured on OP9 feeders for 11 days. The cultures were harvested, and samples were analyzed by FACS (Day11 cells). All remaining cells were transferred to fresh OP9 plates and cultured for an additional 7 days. These cells were subsequently harvested and analyzed by FACS (Day18 cells).