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. 2014 May 14;9(5):e97335. doi: 10.1371/journal.pone.0097335

Figure 3. T cell receptor typing.

Figure 3

CD34+ cells were affinity purified from Day18 cultures as described in the text and cultured on OP9-DL4. After 33 days of co-culture, the cells were analyzed by FACS. a, T cells derived from hiPSC-21 were typed for TCRVβ with the Beta Mark TCR Repertoire Kit from Beckman Coulter. The kit contains eight vials. Each vial contains 3 monoclonal antibodies that recognize 3 different Vβ chains. The antibodies were conjugated with FITC, PE or FITC plus PE. A total 24 different mAbs in this kit can detect 24 Vβ chains (belonging to 19 different families). The cells were gated on the CD3+ populations. b, TCRVγ, TCRVδ and NKG2D expression were examined in T cells derived from hiPSC-19 and hiPSC-21. The data were gated on CD3+ and TCR-γδ+ populations.