Table 1. Highly diverse CDR3 regions in populations of T cells derived in vitro from hiPSC.

Vβ	(D) region	Jβ	Vβ	Jβ
CTCGGCCGTGTATCTCTGTGCCAGCAGC	ACAGGG	TACAATGAGCAGTTCTTCGGGCCAGGGACAC	V11-2	J2-1
CTCGGCCGTGTATCTCTGTGCCAGCAGCT	ACA	CCTACAATGGGCAGTTCTTCGGGCCAGGGACAC	V11-2	J2-1
CTCGGCCGTGTATCTCTGTGCCAGCAGCTTAGA	GG	ATGAAAAACTGTTTTTTGGCAGTGGAACCCAG	V11-2	J1-4
CTCGGCCGTGTATCTCTGTGCCAGCAGCTTA	CAGGGGGCG	GAAAAACTGTTTTTTGGCAGTGGAACCCAG	V11-2	J1-4
CTCGGCCGTGTATCTCTGTGCCAGCAGC	CAGGG	TGAAAAACTGTTTTTTGGCAGTGGAACCCAG	V11-2	J1-4
CTCGGCCGTGTATCTCTGTGCCAGCAGCTT	CCA	TAATGAAAAACTGTTTTTTGGCAGTGGAACCCAG	V11-2	J1-4
CTCGGCCGTGTATCTCTGTGCCAGCAGCT	*	CCTACGAGCAGTACTTCGGGCCGGGCACCAGG	V11-2	J2-7
CTCGGCCGTGTATCTCTGTGCCAGCAGCTTAG	GG	TACGAGCAGTACTTCGGGCCGGGCACCAGG	V11-2	J2-7
CTCGGCCGTGTATCTCTGTGCCAGCAGCTTAGACA	GGGG	AGAGACCCAGTACTTCGGGCCAGGCACGCG	V11-2	J2-5
CTCGGCCGTGTATCTCTGTGCCAGCAGCT	GGACAGG	AGAGACCCAGTACTTCGGGCCAGGCACGCG	V11-2	J2-5
CTCGGCCGTGTATCTCTGTGCCAGCAGCT	ACAGGG	AAGAGACCCAGTACTTCGGGCCAGGCACGCG	V11-2	J2-5
CTCGGCCGTGTATCTCTGTGCCAGCAGCTTA	CAGGGGGCG	AACTATGGCTACACCTTCGGTTCGGGGACCAGGT	V11-2	J1-2
TTGGGGACTCGGCCATGTATCTCTGTGCCAGCAGC	CAGGG	TGAAAAACTGTTTTTTGGCAGTGGAACCCAG	V11-1	J1-4
GGACTCGGCCGTGTATCTCTGTGCCAGCAGCTTA	ACTAGCGGGGGGGC	CACAGATACGCAGTATTTTGGCCCAGGCACCCGGC	V7-2	J2-3
GGACTCGGCCGTGTATCTCTGTGCCAGCAGCTTAG	GACAGGG	CTATGGCTACACCTTCGGTTCGGGGACCAGGT	V7-2	J1-2
AGATTCGGCAGCTTATTTCTGTGCCAGCTCACCAC	AGG	ACTATGGCTACACCTTCGGTTCGGGGACCAGGT	V18	J1-2
CACATACCTCTCAGTACCTCTGTGCCAGCAGTGAA	CAGGGGG	ACACTGAAGCTTTCTTTGGACAAGGCACCAGA	V25-1	J1-1
CACATACCTCTCAGTACCTCTGTGCCAGCAGTGA	CAACA	TGAACACTGAAGCTTTCTTTGGACAAGGCACCAGA	V25-1	J1-1
GGAGGACTCAGCCATGTACTTCTGTGCCAGCAGTG	G	GAACACTGAAGCTTTCTTTGGACAAGGCACCAGA	V2	J1-1
GACAGCTTTCTATCTCTGTGCCAGTAGTATAGACA	*	GCTCCTACAATGAGCAGTTCTTCGGGCCAGGGACAC	V19	J2-1

Sequences of 20 TCR-β cDNA clones. Productive rearrangements were detected for 20 of 22 randomly selected clones.

Sequences between Vβ and Jβ were designated D regions. *Clones in which no Vβ and Jβ sequences were detected around the V(D)J junction region.