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. 2014 May 14;9(5):e97485. doi: 10.1371/journal.pone.0097485

Figure 8. MMP-8 expression is increased in peripheral blood monocytes but not in peripheral blood neutrophils.

Figure 8

Neutrophils and monocytes were isolated from blood samples obtained from IPF patients being evaluated for lung transplantation and healthy volunteers. MMP-8 protein levels were measured in neutrophil lysates (A) and monocyte lysates (C) (all prepared at 5×106 cells/ml) using an ELISA. In A and C, data are mean + SEM, n = 6–7 healthy volunteers and n = 4–22 IPF patients. MMP-8 steady-state mRNA levels were measured in RNA isolated from neutrophils (B) and monocytes (D) obtained from 7–9 control subjects and 6–9 IPF patients using a commercial gene expression assay for MMP-8 and 18 S as an endogenous reference. Data are expressed as fold change + SEM in B and as ΔCT in D (CT for MMP-8 - CT for 18 S as MMP-8 transcripts were not detected in 7 out of 9 controls [CT >60 for MMP-8 and plotted as ΔCT >33] or expressed at low levels in monocytes from 2 of the control subjects). Asterisks indicate p<0.001.