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. 2014 May 14;9(5):e97774. doi: 10.1371/journal.pone.0097774

Table 3. Absolute intracellular EGT concentrations ( µM) in S. pombe cells.

Cell condition Culture medium Intracellular EGT ( µM)
WT vegetative EMM2 0.3
WT nitrogen starvation EMM2-N (24 h) 157.4
WT glucose starvation EMM2-LG (24 h) 41.6
P81nmt1-egt1+ EMM2 32.4
P41nmt1-egt1+ EMM2 181.2
P3nmt1-egt1+ EMM2 1606.3

Intracellular concentrations were derived from measured normalized peak areas using a calibration curve generated by injections of pure EGT in 10-fold dilution steps. The detailed calculation method is described in Figure S6.