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. 2014 May 14;9(5):e97579. doi: 10.1371/journal.pone.0097579

Figure 1. HBV-ZFN target sites.

Figure 1

(a) HBV rcDNA genome map showing HBV ORFs and ZFN target site locations. (b) HBV target site sequence heterogeneity for HBV-ZFN pairs 1–3 across 3847 complete HBV genotype A–H sequences found in Genbank. For each ZFN pair, the target sequence, consensus sequence logo plot and nucleotide Rate4Site (R4S) scores are shown. ZFN spacer nucleotides are highlighted in red and divergent nucleotides between the ZFN target site and the consensus sequence are bold and underlined. Rate4Site scores are graded from low (white) to high (black) sequence heterogeneity. Single nucleotide polymorphisms present in the HepAD38 genomic HBV sequence are shown above each target site in blue. ORF – open reading frame; R4S – Rate4Site; ZFN – zinc finger nuclease.