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. 2014 May 14;9(5):e97478. doi: 10.1371/journal.pone.0097478

Figure 2. Recognition of Env proteins from different constructs by the selected scFv.

Figure 2

(A) Binding of the two antibody classes scFv A7 and A2 to gp140 and gp120 Env proteins determined by ELISA. Detection was with anti c-myc antibody (300 ng/well) and HRP conjugated anti-mouse antibody (1∶1,000). (B) Reactivity of scFv-Fc A2 with SDS and DTT treated and non-treated gp41 Env proteins by ELISA. Each sample was tested in triplicates and error bars represent standard deviations of the mean. Significance analysis was performed with two way ANOVA Tukeýs multiple comparison test. (D) Immunoprecipitation of gp140 ADA.C1 protein from culture supernatants on scFv A2 coupled beads. Fractions of scFv A2 and gp140 flow through (FT), washing (W1-W4), elution (E1–E3) and control resin were analyzed by Western blot with HIV-positive serum (upper panel) and trimer-specific mAb Md-1 (lower panel).