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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Invest Dermatol. 2013 Dec 10;134(6):1710–1717. doi: 10.1038/jid.2013.530

Figure 3. TLR4 deficiency augments IL-12 and IL-23 mRNA expression in mouse BMDC.

Figure 3

BMDC were prepared as described in the Methods section. BMDC were exposed to acute UVB (3 mJ/cm2) radiation and thereafter harvested at 0-48 h. BMDC were obtained and analyzed for IL-12p35 and IL-23p19 expression as mentioned in the Methods section. There was an increase in (a) IL-12p35 and (b) IL-23p19 mRNA expression with increase in time post UV exposure as determined by quantitative real time PCR (qPCR), and this increase was more prominent in the BMDC of TLR4-/- mice than in the UV-exposed BMDC of TLR4+/+ mice. Similar results were obtained for (c) IL-12p35 and (d) IL-23p19 protein expression by ELISA. Experiments were conducted and repeated separately in triplicates in each group with identical results. Scale bar = 50 μm. The experiments were repeated twice with similar results. (**p<0.01 and ***p<0.001)