Table 1.

Pre-steady-state rate constants for incorporation of EFdA-TP or dATP by RT and pol γ.^a

Enzyme	Nucleotide or analog	kpol (s−1)	Kd (μM)	Efficiencyb (μM−1 s−1)	Discriminationc
RT	dATP	8.0 ± 0.7	3.8 ± 0.8	2.1	0.47
	EFdA-TP	5.8 ± 0.3	1.3 ± 0.2	4.5
pol γd	dATP	220 ± 16	3.2 ± 0.7	69	4,300
	EFdA-TP	0.29 ± 0.02	18 ± 4	0.016

^aKinetic parameters determined from at least eight (RT), or at least seven (pol γ) single turnover experiments with varying dATP or EFdA-TP concentrations.

^bEfficiency = k_pol/K_d

^c $\text{Discrimination}=\frac{\text{efficiencydATP}}{\text{efficiencyEFdA}}$

^d(Sohl et al., 2012b)