Table 2.

Pre-steady-state rate constants for post-EFdA incorporation of a single dCTP (the next correct nucleotide) by RT and pol γ^a.

Enzyme	kpol (s−1)	Kd (μM)	Efficiencyb (μM−1 s−1)
RT	0.0064 ±0.0004	1.9 ± 0.4	0.0034c
pol γ	≤0.0002d	N. D.	N. D.

^aKinetic parameters determined from nine (RT) or seven (pol γ) single turnover experiments with varying dCTP concentrations.

^bN.D.: not determined.

^cIncorporation of dCTP after AMP in a similar primer/template has a measured efficiency of 1.5 μM⁻¹ s⁻¹ (Kim et al., 2012).

^dIncorporation of dCTP after AMP in a similar primer/template has a measured k_pol of 72 ± 3 s⁻¹ (Sohl et al., 2013).