Table 2.
Co-localization coefficients to assess level of co-localization of caveolin-1 (Cav-1, red), plasmalemmal vesicle-associated protein-1 (PV-1, green), and fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA, blue) in cultured mouse brain endothelial cells treated with medium alone (control), 4 mg/ml of Fg, (Fg), 4 mg/ml of Fg in the presence of 12 ng/ml tissue inhibitor of metalloproteinases-4 (Fg-TIMP4), and with TIMP-4 alone (TIMP4).
| Co-localization | Control | Fg | Fg-TIMP4 | TIMP4 |
|---|---|---|---|---|
| Cav-1/PV-1 (red/green) | ||||
| Pearson’s correlation Rr | 0.74 | 0.79 | 0.71 | 0.75 |
| Overlap coefficient R | 0.78 | 0.83 | 0.75 | 0.78 |
| Overlap coefficient k2 | 0.57 | 0.8 | 0.47 | 0.6 |
| Co-localization (ch1>0) m2 | 0.76 | 0.82 | 0.7 | 0.75 |
| Cav-1/BSA (red/blue) | ||||
| Pearson’s correlation Rr | 0.09 | 0.16 | 0.09 | 0.09 |
| Overlap coefficient R | 0.15 | 0.25 | 0.16 | 0.16 |
| Overlap coefficient k2 | 0.14 | 0.4 | 0.2 | 0.14 |
| Co-localization (ch1>0) m2 | 0.47 | 0.6 | 0.46 | 0.43 |
| PV-1/BSA (green/blue) | ||||
| Pearson’s correlation Rr | 0.11 | 0.23 | 0.14 | 0.13 |
| Overlap coefficient R | 0.19 | 0.33 | 0.22 | 0.2 |
| Overlap coefficient k2 | 0.24 | 0.7 | 0.4 | 0.22 |
| Co-localization (ch1>0) m2 | 0.68 | 0.9 | 0.73 | 0.64 |