Table 1. Microarray data.
List of genes commonly regulated by 1,25D (100nM/24h) in all VDR positive cells (WT145 cells and three KOhVDR clones). None of these genes were significantly altered by 1,25D treatment in KO240 cells. Shown are fold change for each cell line (treated/control) calculated from the array dataset. Validation of a subset of these genes by real time PCR is shown in Figures 5 and 6.
| Fold Change (100nM 1,25D/Con) | |||||
|---|---|---|---|---|---|
| Gene | KO240 | WT145 | KOhVDR1 | KOhVDR2 | KOhVDR4 |
| A930018M24RK | 1.2 | 5.5 | 7.4 | 6.0 | 6.5 |
| Abcd2 | 1.0 | 2.2 | 2.1 | 1.8 | 2.2 |
| Apcdd1 | 1.1 | 3.8 | 2.1 | 2.6 | 1.9 |
| C3 | 1.2 | 2.1 | 2.7 | 2.0 | 1.7 |
| Cib2 | 1.0 | 4.6 | 9.9 | 8.0 | 9.5 |
| Ckb | 1.3 | 2.2 | 3.4 | 3.2 | 4.0 |
| Crabp2 | 1.0 | 4.0 | 2.6 | 2.6 | 4.3 |
| Cyp24a1 | 1.1 | 50.3 | 19.4 | 15.9 | 16.0 |
| Elovl7 | 1.0 | 4.3 | 4.5 | 3.4 | 4.4 |
| Enpp1 | 1.0 | 6.7 | 3.8 | 4.3 | 4.5 |
| Fam210b | 1.0 | 2.1 | 2.6 | 2.7 | 1.9 |
| Fbn1 | 1.0 | 2.2 | 2.0 | 2.0 | 2.1 |
| Fst | 1.0 | 2.6 | 2.2 | 1.9 | 2.1 |
| Gpc4 | 1.1 | 2.3 | 2.5 | 2.1 | 2.7 |
| Grk5 | 1.0 | 3.0 | 3.2 | 3.2 | 3.7 |
| Has2 | 0.9 | 0.5 | 0.3 | 0.4 | 0.3 |
| Hbegf | 1.0 | 0.5 | 0.4 | 0.5 | 0.4 |
| Igfbp4 | 1.0 | 2.9 | 3.1 | 3.2 | 6.4 |
| Islr | 0.8 | 4.3 | 5.2 | 2.2 | 3.5 |
| Kltl | 1.0 | 2.5 | 2.8 | 2.8 | 3.0 |
| Kl19 | 0.9 | 2.3 | 3.4 | 2.3 | 2.7 |
| Npr3 | 1.0 | 2.2 | 5.3 | 5.8 | 6.3 |
| Plau | 1.0 | 0.4 | 0.3 | 0.3 | 0.3 |
| Pnp1 | 1.0 | 2.1 | 2.3 | 2.0 | 2.4 |
| Postn | 1.0 | 0.2 | 0.3 | 0.3 | 0.4 |
| Prelp | 0.9 | 5.2 | 6.3 | 6.3 | 8.8 |
| Rassf2 | 1.0 | 3.4 | 5.0 | 3.3 | 4.1 |
| Relt | 1.0 | 2.4 | 2.5 | 2.5 | 2.6 |
| Rgl1 | 0.9 | 2.5 | 1.8 | 2.5 | 2.3 |
| Sepp1 | 1.0 | 3.4 | 5.7 | 4.0 | 6.1 |
| Serpinb9b | 0.9 | 2.0 | 2.3 | 3.0 | 3.0 |
| Slfn2 | 0.9 | 2.4 | 2.4 | 1.8 | 2.3 |
| Sulf2 | 1.0 | 2.9 | 3.3 | 2.5 | 2.6 |
| Tgm2 | 1.0 | 2.0 | 4.9 | 4.9 | 5.3 |
| Tspan2 | 1.0 | 2.3 | 4.9 | 3.6 | 4.1 |