Table 3.
Effect of TATA-binding protein on the transcription efficiency
| Template | Reaction | Signal (RLU average) | % RNA synthesis* |
% RNA synthesis |
|---|---|---|---|---|
| TATA mutant vs. standard** | ||||
| HS-DNA_std |
Positive |
1306740 |
100% ± 3% |
100% ± 3% |
| Negative |
9768 |
|
|
|
| α-amanitin 200 nM |
161585 |
12% ± 1% |
|
|
| HS-DNA_mut | Positive |
985650 |
100% ± 10% |
75% ± 7% |
| Negative |
11795 |
|
|
|
| α-amanitin 200 nM | 130255 | 12% ± 1% |
Transcription reactions were performed using the standard DNA template (HS-DNA_std) or a TATA-box mutated template (HS-DNA_mut). Transcription efficacy was determined by QuantiGene quantification.
*Compared to respective average positive control, less the respective negative controls.
**Compared to average positive control on standard template, less the respective negative controls.