Table 1.
Strains and plasmids used in this study
| Name | Relevant genotype | Reference |
|---|---|---|
|
Plasmids |
|
|
| p426TEF |
URA3 |
[32] |
| YIplac128 |
LEU2 |
[33] |
| YIplac211 |
URA3 |
[33] |
| YIpDR1 |
YIplac128:TDH3p-GXF1-CYC1t |
[34] |
| YIpDR7 |
YIplac211:TDH3p-XYL1(N272D)-ADH1t; PGK1p-XYL2-PGK1t |
[35] |
| YIpBB1 |
YIplac128:TDH3p-FRD1-CYC1t |
This study |
| YIpBB2 |
YIplac128:TDH3p-OSM1-CYC1t |
This study |
| YIpBB3 |
YIplac128:TDH3p-FRD1-CYC1t; TEF1p-ERO1-ERO1t |
This study |
| YIpBB4 |
YIplac128:TDH3p-OSM1-CYC1t; TEF1p-ERO1-ERO1t |
This study |
|
Yeast strains |
|
|
| TMB3043 |
CEN.PK2-1C; gre3-Δ; his3::PGK1p-XKS1-PGK1t, HIS3; tal1::PGK1p-TAL1-PGK1t; tkl1::PGK1p-TKL1-PGK1t; rki1::PGK1p-RKI1-PGK1t; rpe1::PGK1p-RPE1-PGK1t; ura3, leu2 |
[36] |
| TMB3452 |
TMB3043; ura3::YIpDR7; leu2 |
This study |
| TMB3455 |
TMB3452; leu2::YIplac128 |
This study |
| TMB3456 |
TMB3452; leu2::YIpBB1 |
This study |
| TMB3457 |
TMB3452; leu2::YIpBB2 |
This study |
| TMB3458 |
TMB3452; leu2::YIpBB3 |
This study |
| TMB3459 | TMB3452; leu2::YIpBB4 | This study |