Table 2.
Maximum specific growth rates and product yields in anaerobic batch fermentation of 20 g/L glucose and 50 g/L xylose in defined medium (2X YNB) using strains TMB3455, TMB3457 and TMB3459
| |
Glucose phase |
Xylose phase |
||||
|---|---|---|---|---|---|---|
| TMB3455 (control) | TMB3457 ( OSM1 ) | TMB3459 ( OSM1 ERO1 ) | TMB3455 (control) | TMB3457 ( OSM1 ) | TMB3459 ( OSM1 ERO1 ) | |
|
μmax (1/h) |
0.29 ± 0.012 |
0.30 ± 0.019 |
0.32 ± 0.044 |
0.029 ± 0.002 |
0.018 ± 0.001 |
0.028 ± 0.003 |
|
Yields per substrate (mol/mol sugar) | ||||||
|
Y
xylt/s
|
– |
– |
– |
0.24 ± 0.03 |
0.25 ± 0.01 |
0.20 ± 0.02 |
|
Y
glyc/s
|
0.22 ± 0.02 |
0.20 ± 0.02 |
0.20 ± 0.05 |
0.020 ± 0.000 |
0.016 ± 0.002 |
0.012 ± 0.001 |
|
Y
ac/s
|
0.020 ± 0.001 |
0.012 ± 0.000 |
0.017 ± 0.005 |
0.019 ± 0.001 |
0.031 ± 0.003 |
0.029 ± 0.003 |
|
Y
etoh/s
|
1.87 ± 0.14 |
1.96 ± 0.21 |
1.96 ± 0.37 |
1.03 ± 0.00 |
1.24 ± 0.15 |
0.87 ± 0.00 |
|
Y
succ/s
|
0.003 ± 0.000 |
0.001 ± 0.000 |
0.002 ± 0.001 |
0.009 ± 0.000 |
0.007 ± 0.002 |
0.006 ± 0.000 |
|
Yx/s (g/mol) |
10.4 ± 0.5 |
10.5 ± 0.2 |
11.9 ± 0.9 |
3.8 ± 0.4 |
2.7 ± 0.6 |
3.9 ± 0.4 |
|
Yields per biomass (mmol/g CDW) | ||||||
|
Y
xylt/x
|
– |
– |
– |
63.1 ± 0.3 |
92.9 ± 25.8 |
50.3 ± 0.8 |
|
Y
glyc/x
|
20.2 ± 0.8 |
18.3 ± 0.5 |
16.6 ± 0.2 |
5.2 ± 0.6 |
5.8 ± 0.7 |
3.2 ± 0.6 |
|
Y
ac/x
|
2.1 ± 0.1 |
1.8 ± 0.1 |
1.8 ± 0.0 |
5.1 ± 0.3 |
11.7 ± 1.8 |
7.3 ± 0.0 |
|
Y
etoh/x
|
153 ± 8 |
153 ± 11 |
147 ± 7 |
271 ± 30 |
458 ± 53 |
223 ± 23 |
| Y succ/x | 0.19 ± 0.04 | 0.28 ± 0.18 | 0.22 ± 0.04 | 2.5 ± 0.4 | 2.4 ± 0.3 | 1.6 ± 0.22 |
Values are given as mean ± standard deviation of two independent experiments. Data for strains TMB3456 and TMB3458 is reported in Additional file 2: Table S3.