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. 2014 Apr 2;14:87. doi: 10.1186/1471-2229-14-87

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Copyright © 2014 Pilati et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PnLOXA localization demonstrated by the expression of YFP fusion constructs in grapevine leaves. Leaves were infiltrated with Agrobacterium tumefaciens carrying the pGreen[PnLOXAtransitpeptide_1-47-YFP] and pGreen[PnLOXAtransitpeptidePLAT_1-220-YFP] constructs. Chlorophyll (Chl) and YFP fluorescence were recorded using Leica SP II confocal microscope. Merge is the computed overlay of the two fluorescence images. Reference bar is 10 μm.