Figure 1.

Different types of preparations used to record CMMC activity in isolated whole segments of mouse colon. (A) Preparation 1 is a novel preparation, where video recordings are made from the empty whole colon, with only a single pin inserted at proximal and distal end to anchor the preparation to the colon. No force transducer clips pinch the bowel. (B) Shows preparation 2, where the colon has force transducer clips attached to the serosal surface, but no resting tension is applied to the colon. (C) Preparation 3, shows the colon tied to an infusion bath with suture thread. (D) Preparation 4 shows the oral and anal ends of the colon tied (as in preparation 3), but this preparation is also pierced with hooks and attached to force transducer under 1 gm resting tension.