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. 2014 Apr 30;14:304. doi: 10.1186/1471-2407-14-304

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Copyright © 2014 Cortes-Dericks et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cisplatin + DEAB-resistant cells show increased mRNA levels of ALDH isozymes and CD44. mRNA was isolated from non-treated and surviving cells of the ALDH-sorted fractions of H28, H2052 and Meso4 after cisplatin treatment in the presence or absence of DEAB for 48 and 72 h. Relative mRNA expression levels of ALDH1A2 and ALDH1A3 (A-C) and CD44 (D-F) were determined by qRT-PCR using the ΔΔCT method. Histograms represent the means and SDs from 3 independent experiments each. Results are statistically significant if p <0.05 (*p <0.05, ***p <0.001).