Table 1.
Sphere-forming efficiency of the three MPM cell lines
| Cell line | Sphere generation | Sphere size (μm) | Sphere number/SD | Sphere efficiency (%) |
|---|---|---|---|---|
| H28 |
1° |
50 - 120 |
51/17.8 |
1.0 |
| |
2° |
40 - 100 |
47.7/4.9 |
0.95 |
| |
3° |
40 - 100 |
20/5.0 |
0.40 |
| H2052 |
1° |
100 - 600 |
11.5/5.7 |
0.23 |
| |
2° |
120 - 500 |
20/6.2 |
0.40 |
| |
3° |
100 - 500 |
25/7.4 |
0.50 |
| Meso4 |
1° |
80 - 600 |
14/5.7 |
0.28 |
| |
2° |
40 – 500 |
12.5/5.3 |
0.25 |
| 3° | 40 - 500 | 10/5.0 | 0.20 |
Single-cell suspensions of H28, H2052 and Meso4 were seeded at 5 x 103/well/ml onto a 24-well plate. Sphere-forming efficiency (%) was determined by dividing the number of spheres formed by the original seeded cells. The quotient was then multiplied by 100.