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UPGMA clustering based on weighted (A) and unweighted UniFrac (B) distance analysis. 250 mg of stool (N = 8) was collected from the outer (LO) and inner (LI) layers and after the stool had been homogenised (LH). Stools were collected in duplicates for each condition (48 samples in total). Unweighted UniFrac allows clustering by taking into account only the microbial composition, while weighted UniFrac considers both composition and abundance of OTUs.
