Figure 1.

Cell migration and invasion assays. (A) Representative images from the scratch assays showing the migration of SHY_vector, SHY_TG2, and SHY_mutant cells. Mytomycin C treated cells were preincubated with resveratrol (1 μM and 10 μM) for 24 h; a scratch was made and further incubated with resveratrol. At 0 h and 48 h after scratch, cells were photographed. Dotted lines represent the edge of migrated cells in the scratch. Upper right panel: Western blot for TG2 protein using 10 μg total cell extract from SHY_vector, SHY_TG2, SHY_mutant, Panc-28, and Hs766T cells. (B) The distance covered by cells in the original empty area was measured and plotted in %. Bars represent mean ± SD of three independent experiments. *p value < 0.05. (C-E) Images from collagen-transwell and matrigel-transwell assays. After 48 h with or without resveratrol treatment, cells were trypsinized and seeded on collagen-transwell (C) or matrigel-transwell inserts in the presence of resveratrol (E). After 15 h, migrated cells on the lower side of inserts were stained with Hema-3 stain (arrow), counted from ten random fields and plotted (D and F). Bars are mean ± SD of at least three independent experiments and *p value < 0.05. (G) Bar diagram represents the migration of Panc-28 and Hs766T cells in scratch assays in the presence of resveratrol as performed with SH-SY5Y cells. Migrated cells into the original empty area were photographed and plotted. Bars are mean ± SD of three independent experiments. *p value < 0.05. (H) Migration and invasion assays for Panc-28 cells were carried out as with neuroblastoma cells in transwell inserts. Migrated/invaded cells were counted from 10 random fields and plotted. Bars are mean ± SD of three independent experiments. *p value < 0.05.