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. 2014 May 15;4:4983. doi: 10.1038/srep04983

Figure 5. CAT-SKL thwarts ADDL-induced proliferation of peroxisomes.

Figure 5

Primary rat cortical/hippocampal neurons were treated as follows: (a) Vehicle control for 24 hours. (b) ADDLs (1 μM) for 24 hours. (c) CAT-SKL (1 μM) for 24 hours followed by ADDLs (1 μM) for 24 hours. (d) ADDLs (1 μM) for 24 hours followed by CAT-SKL (1 μM) for 24 hours. Neurons were immunostained with anti-PMP70 (70 kDa peroxisomal membrane protein) antibodies followed by AlexaFluor 546 goat anti-rabbit antibodies (red fluorescence). Quantification (inset) was accomplished using Image J shareware. Statistical analysis was performed using one-way ANOVA. When ANOVA indicated significant treatment effects, means were separated using Tukey's Multiple Comparison Test. Bars represent mean ± SEM for replicate samples (n = 5). Means followed by the same letter are not significantly different (p > 0.05). Scale bar = 20 μm.