Fig. 4. Expression of p27, cyclin A and cyclin E in A1/A2-KO MEFs.

(A) Expression of cell cycle inhibitor p27 in representative MEFs from each genotype was determined by RT-PCR and Western blot at culture passage 4. γ-tubulin was used as loading control and β-actin gene expression was used as internal control. (B) Expression of cyclin A and cyclin E in representative MEFs from each genotype was determined by Western blot at culture passage 4. Vinculin was used as loading control.