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. 2014 Mar 31;13:74. doi: 10.1186/1476-4598-13-74

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Copyright © 2014 Alaña et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Modulation of HES1 and HEY1 expression by Notch signaling and PTOV1 in prostate cell lines. (A) The transcription of HES1 and HEY1 genes in RWPE1 and PC-3 cells is modulated by the γ-secretase inhibitor DAPT. Cells were treated with DAPT or solvent (CTL) for 4 days and HES1 or HEY1 transcript levels quantified by real-time RT-PCR. Right: The HES1 promoter is modulated by DAPT and by the negative dominant-MAML1 (dnMAML1). PC-3 cells, transfected with HES-luciferase and TK-Renilla, were either treated with DAPT or co-transfected with ICN and ICN plus dnMAML1 and firefly luciferase activity, normalized to Renilla, determined. (B) Knockdown of PTOV1 in RWPE and PC-3 prostate cells by shRNAs causes the upregulation of HES1 and HEY1 mRNA. Cells were transduced with shRNA1397 and shRNA1439 lentiviruses and analyzed by real-time RT-PCR. Values were normalized to RPS14 and for relative values in cells bearing control shRNA.