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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: J Immunol. 2014 Apr 7;192(10):4821–4832. doi: 10.4049/jimmunol.1302980

Figure 1. SARM is required for optimal TLR-induced CCL5 expression, but dispensable for TNF.

Figure 1

Primary WT and Sarm−/− BMDMs were stimulated with 100ng/ml LPS (A,C,E,G) or 5μg/ml CLO75 (B,D,F,H) for the indicated times, or medium as control. Ccl5 (A,B) or Tnf (E,F) mRNA were assayed by quantitative RT-PCR, normalized to the housekeeping gene β-actin and are presented relative to the untreated WT control. Supernatants were assayed for CCL5 (C,D) or TNF (G,H) protein by ELISA. I, Peritoneal macrophages were generated from WT and Sarm−/− mice and stimulated with 10μg/ml CL075 for 24h. Supernatants were assayed for CCL5 by ELISA. The data are mean ± SD of triplicate samples and are representative of at least three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with WT (Student's t-test).