. Author manuscript; available in PMC: 2015 May 15.

Published in final edited form as: J Immunol. 2014 Apr 7;192(10):4821–4832. doi: 10.4049/jimmunol.1302980

Table 1.

PCR primer sequences

Target gene	Forward primer (5’-3’)	Reverse primer (5’-3’)
β-actin mRNA	TCCAGCCTTCCTTCTTGGGT	GCACTGTGTTGGCATAGAGGTC
Ccl5 mRNA	CTCACCATATGGCTCGGACA	ACAAACACGACTGCAAGATTGG
Tnf mRNA	TCCCCAAAGGGATGAGAAGTT	GTTTGCTACGACGTGGGCTAC
Il1b mRNA	GTGAAATGCCACCTTTTGACAGTGATGAG	CTGCTGCGAGATTTGAAGCTGGATG
Ccl2 mRNA	AACTGCATCTGCCCT	ACGGGTCAACTTCAC
Cxcl10 mRNA	TCTGAGTGGGACTCAAGGGAT	TCGTGGCAATGATCTCAACACG
Ifit2 mRNA	GACTTAGAGGTGCTGCACAG	CTCGTTGTACTCATGACTGCTG
Irf7 mRNA	TTGGATCTACTGTGGGCCCA	CTTGCCAGAAATGATCCTGGG
Ccl5 pre-mRNA¹	GTGTTGACCTTCCTCTCTCC	CCTCTATCCTAGCTCATCTCC
Ccl5 mRNA¹	CTTGCAGTCGTGTTTGTCACTC	CCTCTATCCTAGCTCATCTCC
Ccl5 promoter (ChIP)	GCAGTTAGAGGCAGAGTCATAC	CCAGGGTAGCAGAGGAAGTG
Tnf promoter (ChIP)	GATTCCTTGATGCCTGGGTG	GCTCTCATTCAACCCTCGGA
total Sarm mRNA	CGCTGCCCTGTACTGGAGG	CTTCAGGAGGCTGGCCAGCT
Sarm_724/_764 mRNA	CCTTCGCCAGCTACGCTACTTG	CTTCAGGAGGCTGGCCAGCT
wt Sarm locus (Genotyping)	ACGCCTGGTTTCTTACTCTACGA	GCTGGGGCCTCCTTACCTCTT
mutated Sarm locus (Genotyping)	CAGGTAGCCGGATCAAGCGTATGC	CCTGTCCGGTGCCCTGAATGAACT

To distinguish unspliced transcripts (pre-mRNA) and spliced transcripts (mRNA), the forward primer was either designed to bind within an intron (unspliced) or intron spanning (spliced), while the reverse primer targeted an exon.