Table 1.
Metabolites with significant differences between mycelia of A. flavus grown in media with or without D-glucal (40 mg/mL)
| Compounds a |
Relative peak area
b
|
Fold increase
c
|
P
value
d
|
|
|---|---|---|---|---|
| Control | D-glucal | |||
|
Organic acids |
|
|
|
|
| Furanacetic acid |
0.0184 ± 0.0039 |
2.9291 ± 0.2771 |
159.10 |
<0.01 |
| Kojic acid |
0.0942 ± 0.0333 |
0.2076 ± 0.0293 |
2.20 |
<0.01 |
|
Sugar metabolism |
|
|
|
|
| Ribitol |
0.0066 ± 0.0038 |
0.0168 ± 0.0051 |
2.56 |
<0.01 |
| Glycerol |
0.0219 ± 0.0055 |
0.0514 ± 0.0350 |
2.34 |
<0.01 |
| D-glucose |
0.0133 ± 0.0060 |
0.1233 ± 0.0400 |
9.27 |
<0.01 |
| D-galactose |
0.0317 ± 0.0096 |
0.1750 ± 0.0743 |
5.53 |
<0.01 |
|
TCA intermediates |
|
|
|
|
| Succinic acid |
0.0053 ± 0.0016 |
0.0020 ± 0.0005 |
0.37 |
<0.01 |
| Malic acid |
0.0023 ± 0.0013 |
ND |
ND |
ND |
| Fumaric acid |
0.0003 ± 0.0001 |
0.0002 ± 0.0000 |
0.53 |
<0.01 |
|
Fatty acids |
|
|
|
|
| Palmitic acid |
0.1428 ± 0.0116 |
0.0856 ± 0.0144 |
0.60 |
<0.01 |
| Stearic acid |
0.0702 ± 0.0150 |
0.0468 ± 0.0072 |
0.66 |
<0.01 |
| Oleic acid |
0.1957 ± 0.0159 |
0.0377 ± 0.0093 |
0.19 |
<0.01 |
| Linoleic acid |
0.2647 ± 0.0219 |
0.1281 ± 0.0212 |
0.48 |
<0.01 |
|
Others |
|
|
|
|
| Glycine |
0.0010 ± 0.0004 |
0.0004 ± 0.0002 |
0.39 |
<0.01 |
| Pyrimidine | 0.0018 ± 0.0005 | 0.0009 ± 0.0001 | 0.53 | <0.01 |
aIndividual metabolites were identified by GC-TOF MS, as described in the Methods. bRelative peak area as normalized to the peak area of heptadecanoic acid. cFolds represents the relative peak areas in D-glucal-treated samples/peak areas in the control. dStatistical differences between control and D-glucal treated samples were calculated by two-tailed Student’s t-test. N.D.: not detected.