Figure 1.

Gating of Dendritic cells in BALF. Total cells were gated in a FSC/SSC-plot (first column). In this cell population, lineage negative/dim (lin^neg/dim) cells were gated (second column). In the lin^neg/dim gate, cells expressing HLA-DR and CD11c were identified as mDCs (lin^neg/dimHLA-DR⁺CD11c⁺)(third column), and cells expressing HLA-DR and CD123 were identified as pDCs (lin^neg/dimHLA-DR⁺CD123⁺)(fourth column). The figure shows the BALF from a patient with COPD (upper panel) and a healthy never-smoker (lower panel).