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. 2014 Jun;28(6):2632–2644. doi: 10.1096/fj.13-246868

Figure 3.

Figure 3.

Effects of TM peptides on formation of SCTR-AT1aR heterocomplexes. A, B) SCTR-Rlu- and AT1aR-YFP-cotransfected cells were treated with different SCTR TM peptides (STM-I to STM-VII; A) and AT1aR TM peptides (ATM-1 to ATM-7; B). The net BRET ratios were significantly reduced by incubation with STM-II, STM-IV, ATM1, or ATM4. C, D) Effects of TM peptides on heteromer formation were confirmed by saturation BRET studies. C) Saturation BRET signals were significantly reduced by STM-II and STM-IV, but not by the mutant forms of these peptides nor STM-VI. D) Similarly, ATM-1 and ATM-4 peptides, but neither their mutant forms nor ATM-3, could reduce BRET signals in the saturation curves. Data are presented as means ± se of data from ≥4 independent experiments performed in duplicate. *P < 0.01 vs. control (no peptide). E) Predicted helical wheels and the peptide sequences of the TM peptides used. Sites of amino acid substitutions of the mutant peptides are highlighted in red. Asterisk indicates the first residue in the helix. Hydrophobic, polar, and positively charged residues are in black, blue, and green, respectively.