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. 2014 Jun;28(6):2478–2491. doi: 10.1096/fj.13-247015

Figure 3.

Figure 3.

hBVR-based KYCCSRK stimulates glucose uptake in cells and enhances the effect of insulin or IGF-1. Stimulation of glucose uptake by KYCCSRK is neither cell-type nor insulin specific. All cells were serum starved overnight and, where indicated, treated with 20 μM myristoyl-KYCCSRK for 2 h prior to treatment with insulin or IGF-1. A) HEK cells: insulin was added to 100 nM for 15 min prior to measuring [3H]-deoxyglucose uptake (see Materials and Methods). B) HEK cells, with or without 40 ng/ml IGF-1 for 15 min. C) HepG2 cells, 100 nM insulin, as in A. D) Human pulmonary artery smooth muscle (PASM) cells, 40 ng/ml IGF-1 as in B. E) Human skeletal muscle cells, insulin-treated, as in A. *P < 0.05, **P < 0.01, ***P < 0.001; ANOVA.