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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: J Med Chem. 2012 Dec 17;55(24):11052–11061. doi: 10.1021/jm301645g

Figure 3.

Figure 3

Role of cell cycle arrest in the anticancer activity of compound 3a and 4a. (A) Total Pt levels of treated A2780 cells were determined after 2 h of drug exposure by ICP-MS. Values given are the relative mean and SD from at least two independent experiments. (B) DNA synthesis levels were determined in A2780 cells by [3H]thymidine incorporation after 24 h of drug treatment at the indicated concentrations. (C) Cell cycle analysis was evaluated by flow cytometry determining the DNA content of PI-stained A2780 cells after 24 h of treatment with the indicated drug concentrations. Percentages of 25 000 cells in G0/G1, S, and G2/M phases of cell cycle were calculated by Cell Quest software.