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. 2014 Apr 6;14:86. doi: 10.1186/1471-2180-14-86

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Copyright © 2014 Rigano et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Las-LAMP reaction optimization. Several temperature, time and primer combinations were applied to Las-LAMP to determine optimal reaction conditions. An aliquot of 10 μl of each Las-LAMP reaction was loaded into a 1.5% agarose gel. After electrophoresis, the gel was stained with ethidium bromide. C^-: negative control without Template. M: 1 Kb plus DNA ladder (Invitrogen).