Table 1.
Specificity of the Las -LAMP assay
|
Species |
Strain |
Detection method |
|
|---|---|---|---|
| Gel | LFD | ||
|
Candidatus Liberibacter asiaticus |
* |
+ |
+ |
|
Xylella fastidiosa |
9a5c |
- |
- |
|
Xanthomonas citri subsp. citri |
306 |
- |
- |
|
Xanthomonas campestris pv. campestris |
8004 |
- |
- |
|
Xanthomonas campestris pv. vesicatoria |
85-10 |
- |
- |
|
Pseudomonas syringae |
DC3000 |
- |
- |
|
Botrytis cinerea |
B-191 |
- |
- |
|
Phytophthora citricola |
* |
- |
- |
|
Guignardia citricarpa |
* |
- |
- |
|
Elsinoe fawcettii |
* |
- |
- |
| Healthy Orange |
N/A |
- |
- |
| Healthy Citrus limon |
N/A |
- |
- |
| Healthy Diaphorina citri | N/A | - | - |
For each sample Las-LAMP reaction was performed in triplicate. Gel: gel electrophoresis. LFD: lateral flow dipstick. +: Positive reaction. -: Negative reaction. *Performed with DNA from an infected plant without symptoms of other disease. N/A: Not applicable.