Table 2.
Comparison between Las -LAMP and real time PCR assay sensitivity from DNA purified from a Candidatus Liberibacter asiaticus positive plant
|
Detection method |
Purified DNA from a
Las
positive citrus plant |
||||||
|---|---|---|---|---|---|---|---|
| 100 ng | 10 ng | 1 ng | 100 pg | 10 pg | 1 pg | 100 fg | |
|
Las-LAMP Gel |
+ |
+ |
+ |
+ |
+ |
- |
- |
|
Las-LAMP FLD |
+ |
+ |
+ |
+ |
+ |
- |
- |
| Real time PCR | + | + | + | + | + | - | - |
For each dilution the Las-LAMP reaction was performed in triplicate. Gel: gel electrophoresis. LFD: lateral flow dipstick. +: Positive reaction. -: Negative reaction. Real time PCR have been scored as positive if amplification could be detected during the reaction time.