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. 2014 May 15;127(10):2339–2350. doi: 10.1242/jcs.146316

Fig. 5.

Fig. 5.

Membrane mobility of Dsc2a increases in the W2A mutant. (A) FRAP of GFP-tagged wild-type and W2A mutant Dsc2a and Dsg2 cadherins at cell–cell contacts showed a significant difference in the recovery rate of Dsc2aW2A and wild-type Dsc2 and Dsg2, whereas mutant Dsg2W2A showed only slight differences. Lyn–GFP is used a reference for proteins that are lipid-anchored to the plasma membrane. Plotted as mean±s.d. (B) Mobile factions and recovery rates (t1/2) are compared using a single exponential curve fitted to the data shown in A. Mobile fractions of Dsc2a–GFP and Dsc2aW2A–GFP are significantly different (P<0.0001), whereas Dsg2–GFP and Dsg2W2A–GFP are less significant (P<0.1). The recovery rates of both pairs are significantly different (P<0.05). Significance was calculated using a comparison of fits, extra sum-of-squares F test.