Figure 4.

Flow cytometric graphs of T-cell lymphoma without fenugreek treatment (A) and when treated with 300 μg/ml of Fenugreek, for 24, and 72 hours, B and C respectively. The very low number of cells migrated to the right- upper panel are the dead cells by apoptosis induction (late apoptosis) the right lower is the early apoptosis indicating that the apoptotic cell cytotoxicity of fenugreek is very low in normal T-cell lymphocytes cell line. Apoptosis and necrosis was measured using Annexin V apoptosis assay kit. (Molecular Probe). The graphical distribution of viable cells, necrotic and population of apoptotic cells was gated according to their staining signals to PI and Annexin V. The data was generated using a FACS caliber flow cytometry.