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. 2014 Apr 15;14:202. doi: 10.1186/1471-2334-14-202

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Copyright © 2014 Sarfo et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Mycolactone concentration in punch biopsies of untreated M. ulcerans disease lesions before antibiotic treatment. (A) Mycolactone concentration in ASL measured by cytotoxicity assay using synthetic mycolactone A/B generated calibration curves. Horizontal lines represent medians and each dot represents one lesion. (B) Mycolactone concentration in punch biopsies of untreated M. ulcerans disease lesions before antibiotic treatment. Mycolactone A/B concentration in ASL measured by multiple reaction monitoring (MRM) methodology with liquid chromatographic separation coupled to a tandem mass spectrometer. Horizontal lines represent medians and each dot represents one lesion.