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. 2014 Apr 18;13:57. doi: 10.1186/1475-2859-13-57

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Copyright © 2014 Várnai et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Endoglucanase production in 3-liter bioreactors with fed-batch cultivation. One clone of each PichiaPink™ recombinant strain was grown in two parallel 3-l fermenters. The SDS-PAGE figures show the accumulation of A)AfCel12A and B)TaCel5A. Sample lanes are marked with the age of the culture in hours for each fermenter. Reactor B of AOX1/AfCel12A is missing from the gel as cells stopped growing in the batch phase. The bands appearing at approx. 24 kDa (panel A) represent AfCel12A; the bands appearing at approx. 37 kDa represent TaCel5A (panel B).