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. 2014 Apr 24;14:287. doi: 10.1186/1471-2407-14-287

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Copyright © 2014 Bodakuntla et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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NEU induces upregulation of vimentin and disrupts polarity in MCF10A breast acini. MCF10A cells were grown as 3D ‘on top’ cultures in Matrigel™. 2, 3 and 5 mM NEU was administered on Day 0 and Day 2. The acini were cultured for 20 days and then immunostained for (A) α6-integrin (green), β-catenin (red) and DAPI (blue) to stain nuclei (B) Vimentin (green) a marker for EMT and (C) E-cadherin (green). The data is representative of 40 – 50 acini from three biologically independent experiments.