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. 2014 Apr 28;14:295. doi: 10.1186/1471-2407-14-295

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Copyright © 2014 Doronin et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Quantitative analysis of the total ganglioside content and proportion of ganglioside GD2. HPTLC analysis of individual gangliosides in EL-4 cells was performed as described in Methods and shown in (A). Ratio of ganglioside GD2 to the total amount of gangliosides in the different tumor cell lines is shown in (B). The total cellular ganglioside content was determined as the sum of individual gangliosides measured by HPTLC densitometry.